ABSTRACT The present study was conducted to investigate the efficacy of oral administration of L. reuteri on growth performance, intestine histomorphology, immunological and gut microbiome of broilers. A total of twenty healthy chickens were used in a five-week experimental trial. Birds were assigned into one of two groups with orally administrated L. reuteri probiotic and without probiotic- (Control -Phosphate-buffered saline). A significant (p<0.05) body weight gain was observed in the chickens in L. reuteri treatment group compare to those in the control group at the end of the trial. In addition, the serum IGF-1 cytokines level significantly enhanced in L. reuteri treatment group. However, there were no notable effects observed on the villus height, crypt depth, muscularis thickness, and submucosal thickness in chickens orally inject with and without L. reuteri. At the phylum level, the presence of Firmicutes (99.5%) was highly abundantin the L. reuteri treatment group. Moreover, the fecal microbial communities of Lactobacillus (99.9%) showed average relative abundance at genus level in L. reuteri treatment group. From this, we concluded that oral administration of L. reuteri would be beneficial to enhance the body weight gain, gut microbiome, and immune status of broiler.

The aim of this research was to assess genetic diversity of Korean-Holstein, Korean Hanwoo, and Ugandan-Holstein dairy cattle. DNA was extracted from either blood or hair of Korean-Holstein (n=74), Korean-Hanwoo (n=75) and Ugandan-Holstein (N=77) using AccuPrep® PCR purification kit. The DNA samples were amplified by multiplex polymerase chain reaction, using GeneTrack M Hanwoo genotyping kit and assayed using ABI genetic analyser 3130XL. Number of alleles, expected heterozygosity (He), observed heterozygosity (Ho), and the polymorphism information content (PIC) were estimated from 10 microsatellite loci in the three breeds. In addition, F-statistics for each of the 10 microsatellites in the three cattle breeds were estimated using fstat version 2.9.3.2 computer program. GENETIX (v.4.02) was used to perform factorial correspondence analysis (FCA) from the allele frequencies and multi-locus clustering was done using STRUCTURE analyses. A total of 124 alleles were detected. The number of alleles per locus varied from eight (TGLA126) to 22 (TGLA122), with an overall mean of 12.2. Expected heterozygosity ranged from 0.617 (SPS115) to 0.854 (TGLA53) and averaged 0.761. Observed heterozygosity ranged from 0.6 (SPS115) to 0.859 (TGLA53); and averaged 0.761. The mean PIC was 0.723; and means of the F-statistics FIT, FST and FIS were 0.077, 0.076 and 0.001 respectively. Although FCA revealed clear differentiation of Uganda-Holstein, Korean-Holstein, and Hanwoo, clustering assignments showed genetic admixture between Ugandan dairy cattle (Uganda-Holstein) and Hanwoo. In conclusion, the allelic variation present at the 10 loci was sufficient to categorize these cattle into distinct breed groups.

The objective of this experiment was to determine the effects of a pure reduction in the dietary crude protein (CP) and metabolic energy (ME) contents on growth performance, nutrient digestibility, blood profile, faecal microflora and odour gas emission in weaned pigs. A total of 80 weaned piglets ((Landrace x Yorkshire) x Duroc) with a mean initial bodyweight (BW) of 6.8 ± 0.5 kg were randomly allotted to four treatments with four replicate pens of five piglets per pen (based on average BW) for 45 days. The dietary treatments consisted of i) CON: basal diet; ii) LME: reduction of 10% of ME in basal diet; iii) LCP: reduction of 10% of CP in basal diet; iv) MECP: reduction of 10% of CP and 10% of ME in basal diet. During the experimental period, average daily feed intake (ADFI) improved in piglets fed the LME and LCP diets, compared with those fed the CON diets. Average daily gain (adG) and gain to feed (G : F) ratio decreased in piglets fed LCP, LME, and MECP diet, when compared with those fed the CON diet. However, during the total experimental period, ADG and G : F ratio were not affected by dietary treatment. With regard to nutrient digestibility, apparent total track digestibility (ATTD) of CP was not affected by experimental diets. The concentration of blood urea nitrogen (BUN) in blood decreased more in piglets fed LME, LCP, and MECP than those fed the CON diet. The emissions of ammonia (NH3), hydrogen sufide (H2S), and volatile fatty acids (VFAs) were lower in piglets fed LME, LCP, and MECP diet than those fed the CON diet. In conclusion, these results indicate that reduction in dietary CP and ME content did not decrease growth performance and nutrient digestibility, and they increased positive effects such as BUN and gas emission reduction.

A novel optical encryption method is proposed in this paper to achieve 3-D image encryption. This proposed encryption algorithm combines the use of computational integral imaging (CII) and linear-complemented maximum-length cellular automata (LC-MLCA) to encrypt a 3D image. In the encryption process, the 2-D elemental image array (EIA) recorded by light rays of the 3-D image are mapped inversely through the lenslet array according the ray tracing theory. Next, the 2-D EIA is encrypted by LC-MLCA algorithm. When decrypting the encrypted image, the 2-D EIA is recovered by the LC-MLCA. Using the computational integral imaging reconstruction (CIIR) technique and a 3-D object is subsequently reconstructed on the output plane from the 2-D recovered EIA. Because the 2-D EIA is composed of a number of elemental images having their own perspectives of a 3-D image, even if the encrypted image is seriously harmed, the 3-D image can be successfully reconstructed only with partial data. To verify the usefulness of the proposed algorithm, we perform computational experiments and present the experimental results for various attacks. The experiments demonstrate that the proposed encryption method is valid and exhibits strong robustness and security.

A deficiência de fósforo disponível nas plantas é considerada o maior fator de limitação na produção de alimentos em diversos solos agrícolas. São necessários recursos minerais para restaurar o conteúdo de fósforo no solo. Em regiões onde fertilizantes convencionais nãosão utilizados devido às limitações de custo ou de seus efeitos ambientais adversos, fontes locais de rocha fosfática estão sendo crescentemente reconhecidas por seu uso potencial como alternativa aos fertilizantes solúveis de fósforo. O principal obstáculo associado ao uso daaplicação direta da rocha fosfática no solo é que o fósforo liberado é, muitas vezes, incapaz de suprir as necessidades das plantas de forma a aumentar a produção. Mecanismos baseados no uso de plantas e micro-organismos são consideradas tecnologias apropriadas de baixo custo, para ativar a solubilização e aumentar a efetividade agronômica das rochas fosfáticas. Mecanismos comuns de dissolução da rocha fosfática, incluindo a produção de prótons e ácidos orgânicos serão revisados tanto para plantas quanto para microorganismos. Esta revisão também tratará das possibilidades para futuras pesquisas com rochas fosfáticas e suas aplicações na agricultura, assim como destaca pesquisas em andamento na Universidade de Guelph, Canadá.

Deficiency in plant-available phosphorus is considered to be a major limiting factor to food production in many agricultural soils. Mineral resources are necessary to restore soil phosphorus content. In regions where conventional fertilizers are not used due to cost limitations or to mitigate adverse environmental effects, local sources of phosphate rock are being increasingly recognized for potential use as alternative phosphorus fertilizers. The main obstacle associated with using directly applied ground phosphate rock is that the phosphate released is often unable to supply sufficient plant-available phosphorus for crop uptake. Plantand microbial-based mechanisms are low-cost, appropriate technologies to enhance the solubilization and increase the agronomic effectiveness of phosphate rock. Common mechanisms of phosphate rock dissolution including proton and organic acid production will be reviewed for both plants and microorganisms. This review will also address possibilities for future research directions and applications to agriculture, as well as highlight ongoing research at the University of Guelph, Guelph, Canada.

Over the last decades, the incidence of ultraviolet B (UVB)-related skin problems has been increasing. Damages induced by UVB radiation are related to mutations that occur as a result of direct DNA damage and/or the production of reactive oxygen species. We investigated the anti-oxidant effects of a Polygonum multiflorum thumb extract against skin damage induced by UVB irradiation. Female SKH-1 hairless mice were divided into three groups: control (N = 7), distilled water- (N = 10), and P. multiflorum extract-treated (PM, N = 10) groups. The PM (10 g) was extracted with 100 mL distilled water, cryo-dried and 9.8 g was obtained. The animals received a topical application of 500 µL distilled water or PM extract (1, 2, 4, 8, and 16%, w/v, dissolved in distilled water) for 30 min after UVB irradiation (wavelength 280-320 nm, 300 mJ/cm²; 3 min) of the dorsal kin for 14 days, and skin immunohistochemistry and Cu,Zn-superoxide dismutase (SOD1) activity were determined. SOD1 immunoreactivity, its protein levels and activities in the skin were significantly reduced by 70% in the distilled water-treated group after UVB irradiation compared to control. However, in the PM extract-treated groups, SOD1 immunoreactivity and its protein and activity levels increased in a dose-dependent manner (1-16%, w/v, PM extract) compared to the distilled water-treated group. SOD1 protein levels and activities in the groups treated with 8 and 16%, w/v, PM extract recovered to 80-90% of the control group levels after UVB. These results suggest that PM extract strongly inhibits the destruction of SOD1 by UV radiation and probably contains anti-skin photoaging agents.